Nicole_FinalEngineering protein-based intracellular delivery systems for macromolecules

Macromolecules such as proteins and nucleic acids can potentially be used as sophisticated therapeutics, reconstituting deficient cellular activity or inhibiting aberrant activity with high specificity. However, while many macromolecules require cytosolic access to function, their entry into the intracellular space is effectively prevented by the cell membrane. I am interested in utilizing naturally-occurring pore-forming proteins as a tool to enable the efficient transport of such molecules across the membrane barrier for potential therapeutic applications.