2018

  1. Rothschilds, AM, Wittrup, KD (2019). What, Why, Where, and When: Bringing Timing to Immuno-Oncology. Trends Immunol40,12-21.
  2. Kauke, MJ, Tisdale, AW, Kelly, RL, Braun, CJ, Hemann, MT, Wittrup, KD (2018). A Raf-Competitive K-Ras Binder Can Fail to Functionally Antagonize Signaling. Mol Cancer Ther17,1773-1780.
  3. Chen, TF, Li, KK, Zhu, EF, Opel, CF, Kauke, MJ, Kim, H, Atolia, E, Wittrup, KD (2018). Artificial Anti-Tumor Opsonizing Proteins with Fibronectin Scaffolds Engineered for Specificity to Each of the Murine FcγR Types. J Mol Biol430,1786-1798.
  4. Angelini, A, Miyabe, Y, Newsted, D, Kwan, BH, Miyabe, C, Kelly, RL, Jamy, MN, Luster, AD, Wittrup, KD (2018). Directed evolution of broadly crossreactive chemokine-blocking antibodies efficacious in arthritis. Nat Commun9,1461.
  5. Kelly, RL, Le, D, Zhao, J, Wittrup, KD (2018). Reduction of Nonspecificity Motifs in Synthetic Antibody Libraries. J Mol Biol430,119-130.
  6. Green, DJ, O'Steen, S, Lin, Y, Comstock, ML, Kenoyer, AL, Hamlin, DK, Wilbur, DS, Fisher, DR, Nartea, M, Hylarides, MD et al. (2018). CD38-bispecific antibody pretargeted radioimmunotherapy for multiple myeloma and other B-cell malignancies. Blood131,611-620.

2017

  1. Kelly, RL, Le, D, Zhao, J, Wittrup, KD (2018). Reduction of Nonspecificity Motifs in Synthetic Antibody Libraries. J Mol Biol430,119-130.
  2. Green, DJ, O'Steen, S, Lin, Y, Comstock, ML, Kenoyer, AL, Hamlin, DK, Wilbur, DS, Fisher, DR, Nartea, M, Hylarides, MD et al. (2018). CD38-bispecific antibody pretargeted radioimmunotherapy for multiple myeloma and other B-cell malignancies. Blood131,611-620.
  3. Kelly, RL, Zhao, J, Le, D, Wittrup, KD (2017). Nonspecificity in a nonimmune human scFv repertoire. MAbs9,1029-1035.
  4. Wittrup, KD (2017). Antitumor Antibodies Can Drive Therapeutic T Cell Responses. Trends Cancer3,615-620.
  5. Kelly, RL, Geoghegan, JC, Feldman, J, Jain, T, Kauke, M, Le, D, Zhao, J, Wittrup, KD (2017). Chaperone proteins as single component reagents to assess antibody nonspecificity. MAbs9,1036-1040.
  6. Kauke, MJ, Traxlmayr, MW, Parker, JA, Kiefer, JD, Knihtila, R, McGee, J, Verdine, G, Mattos, C, Wittrup, KD (2017). An engineered protein antagonist of K-Ras/B-Raf interaction. Sci Rep7,5831.
  7. Cheal, SM, Fung, EK, Patel, M, Xu, H, Guo, HF, Zanzonico, PB, Monette, S, Wittrup, KD, Cheung, NV, Larson, SM et al. (2017). Curative Multicycle Radioimmunotherapy Monitored by Quantitative SPECT/CT-Based Theranostics, Using Bispecific Antibody Pretargeting Strategy in Colorectal Cancer. J Nucl Med58,1735-1742.
  8. Chen, TF, Sazinsky, SL, Houde, D, DiLillo, DJ, Bird, J, Li, KK, Cheng, GT, Qiu, H, Engen, JR, Ravetch, JV et al. (2017). Engineering Aglycosylated IgG Variants with Wild-Type or Improved Binding Affinity to Human Fc Gamma RIIA and Fc Gamma RIIIAs. J Mol Biol429,2528-2541.
  9. Yang, NJ, Kauke, MJ, Sun, F, Yang, LF, Maass, KF, Traxlmayr, MW, Yu, Y, Xu, Y, Langer, RS, Anderson, DG et al. (2017). Cytosolic delivery of siRNA by ultra-high affinity dsRNA binding proteins. Nucleic Acids Res45,7602-7614.
  10. Kwan, BH, Zhu, EF, Tzeng, A, Sugito, HR, Eltahir, AA, Ma, B, Delaney, MK, Murphy, PA, Kauke, MJ, Angelini, A et al. (2017). Integrin-targeted cancer immunotherapy elicits protective adaptive immune responses. J Exp Med214,1679-1690.
  11. Smith, TT, Moffett, HF, Stephan, SB, Opel, CF, Dumigan, AG, Jiang, X, Pillarisetty, VG, Pillai, SPS, Wittrup, KD, Stephan, MT et al. (2017). Biopolymers codelivering engineered T cells and STING agonists can eliminate heterogeneous tumors. J Clin Invest127,2176-2191.
  12. Jain, T, Sun, T, Durand, S, Hall, A, Houston, NR, Nett, JH, Sharkey, B, Bobrowicz, B, Caffry, I, Yu, Y et al. (2017). Biophysical properties of the clinical-stage antibody landscape. Proc Natl Acad Sci U S A114,944-949.
  13. Sharkey, B, Pudi, S, Wallace Moyer, I, Zhong, L, Prinz, B, Baruah, H, Lynaugh, H, Kumar, S, Wittrup, KD, Nett, JH et al. . Purification of common light chain IgG-like bispecific antibodies using highly linear pH gradients. MAbs9,257-268.

2016

  1. Sharkey, B, Pudi, S, Wallace Moyer, I, Zhong, L, Prinz, B, Baruah, H, Lynaugh, H, Kumar, S, Wittrup, KD, Nett, JH et al. . Purification of common light chain IgG-like bispecific antibodies using highly linear pH gradients. MAbs9,257-268.
  2. Tzeng, A, Kauke, MJ, Zhu, EF, Moynihan, KD, Opel, CF, Yang, NJ, Mehta, N, Kelly, RL, Szeto, GL, Overwijk, WW et al. (2016). Temporally Programmed CD8α+ DC Activation Enhances Combination Cancer Immunotherapy. Cell Rep17,2503-2511.
  3. Green, DJ, Frayo, SL, Lin, Y, Hamlin, DK, Fisher, DR, Frost, SH, Kenoyer, AL, Hylarides, MD, Gopal, AK, Gooley, TA et al. (2016). Comparative Analysis of Bispecific Antibody and Streptavidin-Targeted Radioimmunotherapy for B-cell Cancers. Cancer Res76,6669-6679.
  4. Moynihan, KD, Opel, CF, Szeto, GL, Tzeng, A, Zhu, EF, Engreitz, JM, Williams, RT, Rakhra, K, Zhang, MH, Rothschilds, AM et al. (2016). Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses. Nat Med22,1402-1410.
  5. Kelly, RL, Yu, Y, Sun, T, Caffry, I, Lynaugh, H, Brown, M, Jain, T, Xu, Y, Wittrup, KD (2016). Target-independent variable region mediated effects on antibody clearance can be FcRn independent. MAbs8,1269-1275.
  6. Traxlmayr, MW, Kiefer, JD, Srinivas, RR, Lobner, E, Tisdale, AW, Mehta, NK, Yang, NJ, Tidor, B, Wittrup, KD (2016). Strong Enrichment of Aromatic Residues in Binding Sites from a Charge-neutralized Hyperthermostable Sso7d Scaffold Library. J Biol Chem291,22496-22508.
  7. de Picciotto, S, Dickson, PM, Traxlmayr, MW, Marques, BS, Socher, E, Zhao, S, Cheung, S, Kiefer, JD, Wand, AJ, Griffith, LG et al. (2016). Design Principles for SuCESsFul Biosensors: Specific Fluorophore/Analyte Binding and Minimization of Fluorophore/Scaffold Interactions. J Mol Biol428,4228-4241.
  8. Singh, AP, Maass, KF, Betts, AM, Wittrup, KD, Kulkarni, C, King, LE, Khot, A, Shah, DK (2016). Evolution of Antibody-Drug Conjugate Tumor Disposition Model to Predict Preclinical Tumor Pharmacokinetics of Trastuzumab-Emtansine (T-DM1). AAPS J18,861-75.
  9. Bornholdt, ZA, Turner, HL, Murin, CD, Li, W, Sok, D, Souders, CA, Piper, AE, Goff, A, Shamblin, JD, Wollen, SE et al. (2016). Isolation of potent neutralizing antibodies from a survivor of the 2014 Ebola virus outbreak. Science351,1078-83.
  10. Maass, KF, Kulkarni, C, Betts, AM, Wittrup, KD (2016). Determination of Cellular Processing Rates for a Trastuzumab-Maytansinoid Antibody-Drug Conjugate (ADC) Highlights Key Parameters for ADC Design. AAPS J18,635-46.
  11. Schütz, M, Batyuk, A, Klenk, C, Kummer, L, de Picciotto, S, Gülbakan, B, Wu, Y, Newby, GA, Zosel, F, Schöppe, J et al. (2016). Generation of Fluorogen-Activating Designed Ankyrin Repeat Proteins (FADAs) as Versatile Sensor Tools. J Mol Biol428,1272-1289.
  12. Cheal, SM, Xu, H, Guo, HF, Lee, SG, Punzalan, B, Chalasani, S, Fung, EK, Jungbluth, A, Zanzonico, PB, Carrasquillo, JA et al. (2016). Theranostic pretargeted radioimmunotherapy of colorectal cancer xenografts in mice using picomolar affinity ⁸⁶Y- or ¹⁷⁷Lu-DOTA-Bn binding scFv C825/GPA33 IgG bispecific immunoconjugates. Eur J Nucl Med Mol Imaging43,925-937.

2015

  1. Cheal, SM, Xu, H, Guo, HF, Lee, SG, Punzalan, B, Chalasani, S, Fung, EK, Jungbluth, A, Zanzonico, PB, Carrasquillo, JA et al. (2016). Theranostic pretargeted radioimmunotherapy of colorectal cancer xenografts in mice using picomolar affinity ⁸⁶Y- or ¹⁷⁷Lu-DOTA-Bn binding scFv C825/GPA33 IgG bispecific immunoconjugates. Eur J Nucl Med Mol Imaging43,925-937.
  2. Maass, KF, Kulkarni, C, Quadir, MA, Hammond, PT, Betts, AM, Wittrup, KD (2015). A Flow Cytometric Clonogenic Assay Reveals the Single-Cell Potency of Doxorubicin. J Pharm Sci104,4409-4416.
  3. Van Deventer, JA, Kelly, RL, Rajan, S, Wittrup, KD, Sidhu, SS (2015). A switchable yeast display/secretion system. Protein Eng Des Sel28,317-25.
  4. Angelini, A, Chen, TF, de Picciotto, S, Yang, NJ, Tzeng, A, Santos, MS, Van Deventer, JA, Traxlmayr, MW, Wittrup, KD (2015). Protein Engineering and Selection Using Yeast Surface Display. Methods Mol Biol1319,3-36.
  5. Kelly, RL, Sun, T, Jain, T, Caffry, I, Yu, Y, Cao, Y, Lynaugh, H, Brown, M, Vásquez, M, Wittrup, KD et al. (2015). High throughput cross-interaction measures for human IgG1 antibodies correlate with clearance rates in mice. MAbs7,770-7.
  6. Yang, NJ, Liu, DV, Sklaviadis, D, Gui, DY, Vander Heiden, MG, Wittrup, KD (2015). Antibody-mediated neutralization of perfringolysin o for intracellular protein delivery. Mol Pharm12,1992-2000.
  7. Zhu, EF, Gai, SA, Opel, CF, Kwan, BH, Surana, R, Mihm, MC, Kauke, MJ, Moynihan, KD, Angelini, A, Williams, RT et al. (2015). Synergistic innate and adaptive immune response to combination immunotherapy with anti-tumor antigen antibodies and extended serum half-life IL-2. Cancer Cell27,489-501.
  8. Tzeng, A, Kwan, BH, Opel, CF, Navaratna, T, Wittrup, KD (2015). Antigen specificity can be irrelevant to immunocytokine efficacy and biodistribution. Proc Natl Acad Sci U S A112,3320-5.
  9. Wang, S, Mata-Fink, J, Kriegsman, B, Hanson, M, Irvine, DJ, Eisen, HN, Burton, DR, Wittrup, KD, Kardar, M, Chakraborty, AK et al. (2015). Manipulating the selection forces during affinity maturation to generate cross-reactive HIV antibodies. Cell160,785-797.
  10. Rouha, H, Badarau, A, Visram, ZC, Battles, MB, Prinz, B, Magyarics, Z, Nagy, G, Mirkina, I, Stulik, L, Zerbs, M et al. (2015). Five birds, one stone: neutralization of α-hemolysin and 4 bi-component leukocidins of Staphylococcus aureus with a single human monoclonal antibody. MAbs7,243-54.

2014

  1. Paulus, GL, Nelson, JT, Lee, KY, Wang, QH, Reuel, NF, Grassbaugh, BR, Kruss, S, Landry, MP, Kang, JW, Vander Ende, E et al. (2014). A graphene-based physiometer array for the analysis of single biological cells. Sci Rep4,6865.
  2. Yao, X, Williamson, C, Adalsteinsson, VA, D'Agostino, RS, Fitton, T, Smaroff, GG, William, RT, Wittrup, KD, Love, JC (2014). Tumor cells are dislodged into the pulmonary vein during lobectomy. J Thorac Cardiovasc Surg148,3224-31.e1-5.
  3. Liu, DV, Yang, NJ, Wittrup, KD (2014). A Nonpolycationic Fully Proteinaceous Multiagent System for Potent Targeted Delivery of siRNA. Mol Ther Nucleic Acids3,e162.
  4. de Picciotto, S, Imperiali, B, Griffith, LG, Wittrup, KD (2014). Equilibrium and dynamic design principles for binding molecules engineered for reagentless biosensors. Anal Biochem460,9-15.
  5. Yao, X, Choudhury, AD, Yamanaka, YJ, Adalsteinsson, VA, Gierahn, TM, Williamson, CA, Lamb, CR, Taplin, ME, Nakabayashi, M, Chabot, MS et al. (2014). Functional analysis of single cells identifies a rare subset of circulating tumor cells with malignant traits. Integr Biol (Camb)6,388-98.
  6. Van Deventer, JA, Wittrup, KD (2014). Yeast surface display for antibody isolation: library construction, library screening, and affinity maturation. Methods Mol Biol1131,151-81.

2013

  1. Bak, SP, Barnkob, MS, Wittrup, KD, Chen, J (2013). CD8+ T-cell responses rapidly select for antigen-negative tumor cells in the prostate. Cancer Immunol Res1,393-401.
  2. Schmidt, MM, Townson, SA, Andreucci, AJ, King, BM, Schirmer, EB, Murillo, AJ, Dombrowski, C, Tisdale, AW, Lowden, PA, Masci, AL et al. (2013). Crystal structure of an HSA/FcRn complex reveals recycling by competitive mimicry of HSA ligands at a pH-dependent hydrophobic interface. Structure21,1966-78.
  3. Xu, Y, Roach, W, Sun, T, Jain, T, Prinz, B, Yu, TY, Torrey, J, Thomas, J, Bobrowicz, P, Vásquez, M et al. (2013). Addressing polyspecificity of antibodies selected from an in vitro yeast presentation system: a FACS-based, high-throughput selection and analytical tool. Protein Eng Des Sel26,663-70.
  4. Adalsteinsson, VA, Tahirova, N, Tallapragada, N, Yao, X, Campion, L, Angelini, A, Douce, TB, Huang, C, Bowman, B, Williamson, CA et al. (2013). Single cells from human primary colorectal tumors exhibit polyfunctional heterogeneity in secretions of ELR+ CXC chemokines. Integr Biol (Camb)5,1272-81.
  5. Reuel, NF, Grassbaugh, B, Kruss, S, Mundy, JZ, Opel, C, Ogunniyi, AO, Egodage, K, Wahl, R, Helk, B, Zhang, J et al. (2013). Emergent properties of nanosensor arrays: applications for monitoring IgG affinity distributions, weakly affined hypermannosylation, and colony selection for biomanufacturing. ACS Nano7,7472-82.
  6. Pirie, CM, Liu, DV, Wittrup, KD (2013). Targeted cytolysins synergistically potentiate cytoplasmic delivery of gelonin immunotoxin. Mol Cancer Ther12,1774-82.
  7. Yao, X, Labelle, M, Lamb, CR, Dugan, JM, Williamson, CA, Spencer, DR, Christ, KR, Keating, RO, Lee, WD, Paradis, GA et al. (2013). Determination of 35 cell surface antigen levels in malignant pleural effusions identifies CD24 as a marker of disseminated tumor cells. Int J Cancer133,2925-33.
  8. Su, X, Yang, N, Wittrup, KD, Irvine, DJ (2013). Synergistic antitumor activity from two-stage delivery of targeted toxins and endosome-disrupting nanoparticles. Biomacromolecules14,1093-102.
  9. Kwong, B, Gai, SA, Elkhader, J, Wittrup, KD, Irvine, DJ (2013). Localized immunotherapy via liposome-anchored Anti-CD137 + IL-2 prevents lethal toxicity and elicits local and systemic antitumor immunity. Cancer Res73,1547-58.
  10. Chen, TF, de Picciotto, S, Hackel, BJ, Wittrup, KD (2013). Engineering fibronectin-based binding proteins by yeast surface display. Methods Enzymol523,303-26.
  11. Yazaki, PJ, Lee, B, Channappa, D, Cheung, CW, Crow, D, Chea, J, Poku, E, Li, L, Andersen, JT, Sandlie, I et al. (2013). A series of anti-CEA/anti-DOTA bispecific antibody formats evaluated for pre-targeting: comparison of tumor uptake and blood clearance. Protein Eng Des Sel26,187-93.
  12. Mata-Fink, J, Kriegsman, B, Yu, HX, Zhu, H, Hanson, MC, Irvine, DJ, Wittrup, KD (2013). Rapid conformational epitope mapping of anti-gp120 antibodies with a designed mutant panel displayed on yeast. J Mol Biol425,444-56.

2012

  1. Yazaki, PJ, Lee, B, Channappa, D, Cheung, CW, Crow, D, Chea, J, Poku, E, Li, L, Andersen, JT, Sandlie, I et al. (2013). A series of anti-CEA/anti-DOTA bispecific antibody formats evaluated for pre-targeting: comparison of tumor uptake and blood clearance. Protein Eng Des Sel26,187-93.
  2. Mata-Fink, J, Kriegsman, B, Yu, HX, Zhu, H, Hanson, MC, Irvine, DJ, Wittrup, KD (2013). Rapid conformational epitope mapping of anti-gp120 antibodies with a designed mutant panel displayed on yeast. J Mol Biol425,444-56.
  3. Bak, SP, Barnkob, MS, Bai, A, Higham, EM, Wittrup, KD, Chen, J (2012). Differential requirement for CD70 and CD80/CD86 in dendritic cell-mediated activation of tumor-tolerized CD8 T cells. J Immunol189,1708-16.
  4. Spangler, JB, Manzari, MT, Rosalia, EK, Chen, TF, Wittrup, KD (2012). Triepitopic antibody fusions inhibit cetuximab-resistant BRAF and KRAS mutant tumors via EGFR signal repression. J Mol Biol422,532-44.
  5. Orcutt, KD, Rhoden, JJ, Ruiz-Yi, B, Frangioni, JV, Wittrup, KD (2012). Effect of small-molecule-binding affinity on tumor uptake in vivo: a systematic study using a pretargeted bispecific antibody. Mol Cancer Ther11,1365-72.
  6. Wittrup, KD, Verdine, GL (2012). Protein engineering for therapeutics, part B. Preface. Methods Enzymol503,xiii-xiv.
  7. Wittrup, KD, Thurber, GM, Schmidt, MM, Rhoden, JJ (2012). Practical theoretic guidance for the design of tumor-targeting agents. Methods Enzymol503,255-68.
  8. Wittrup, KD, Verdine, GL (2012). Protein engineering for therapeutics, Part A. Preface. Methods Enzymol502,xiii-xiv.
  9. Hackel, BJ, Neil, JR, White, FM, Wittrup, KD (2012). Epidermal growth factor receptor downregulation by small heterodimeric binding proteins. Protein Eng Des Sel25,47-57.
  10. Rhoden, JJ, Wittrup, KD (2012). Dose dependence of intratumoral perivascular distribution of monoclonal antibodies. J Pharm Sci101,860-7.

2011

  1. Hackel, BJ, Neil, JR, White, FM, Wittrup, KD (2012). Epidermal growth factor receptor downregulation by small heterodimeric binding proteins. Protein Eng Des Sel25,47-57.
  2. Rhoden, JJ, Wittrup, KD (2012). Dose dependence of intratumoral perivascular distribution of monoclonal antibodies. J Pharm Sci101,860-7.
  3. Boersma, YL, Chao, G, Steiner, D, Wittrup, KD, Plückthun, A (2011). Bispecific designed ankyrin repeat proteins (DARPins) targeting epidermal growth factor receptor inhibit A431 cell proliferation and receptor recycling. J Biol Chem286,41273-41285.
  4. Schiefner, A, Chatwell, L, Körner, J, Neumaier, I, Colby, DW, Volkmer, R, Wittrup, KD, Skerra, A (2011). A disulfide-free single-domain V(L) intrabody with blocking activity towards huntingtin reveals a novel mode of epitope recognition. J Mol Biol414,337-55.
  5. Ackerman, ME, Lai, JI, Pastan, I, Wittrup, KD (2011). Exploiting bias in a non-immune human antibody library to predict antigenicity. Protein Eng Des Sel24,845-53.
  6. Orcutt, KD, Slusarczyk, AL, Cieslewicz, M, Ruiz-Yi, B, Bhushan, KR, Frangioni, JV, Wittrup, KD (2011). Engineering an antibody with picomolar affinity to DOTA chelates of multiple radionuclides for pretargeted radioimmunotherapy and imaging. Nucl Med Biol38,223-33.
  7. Pirie, CM, Hackel, BJ, Rosenblum, MG, Wittrup, KD (2011). Convergent potency of internalized gelonin immunotoxins across varied cell lines, antigens, and targeting moieties. J Biol Chem286,4165-72.
  8. Swers, JS, Yeung, YA, Wittrup, KD (2011). Integrated mimicry of B cell antibody mutagenesis using yeast homologous recombination. Mol Biotechnol47,57-69.
  9. Orcutt, KD, Nasr, KA, Whitehead, DG, Frangioni, JV, Wittrup, KD (2011). Biodistribution and clearance of small molecule hapten chelates for pretargeted radioimmunotherapy. Mol Imaging Biol13,215-21.

2010

  1. Orcutt, KD, Slusarczyk, AL, Cieslewicz, M, Ruiz-Yi, B, Bhushan, KR, Frangioni, JV, Wittrup, KD (2011). Engineering an antibody with picomolar affinity to DOTA chelates of multiple radionuclides for pretargeted radioimmunotherapy and imaging. Nucl Med Biol38,223-33.
  2. Pirie, CM, Hackel, BJ, Rosenblum, MG, Wittrup, KD (2011). Convergent potency of internalized gelonin immunotoxins across varied cell lines, antigens, and targeting moieties. J Biol Chem286,4165-72.
  3. Spangler, JB, Neil, JR, Abramovitch, S, Yarden, Y, White, FM, Lauffenburger, DA, Wittrup, KD (2010). Combination antibody treatment down-regulates epidermal growth factor receptor by inhibiting endosomal recycling. Proc Natl Acad Sci U S A107,13252-7.
  4. Hackel, BJ, Ackerman, ME, Howland, SW, Wittrup, KD (2010). Stability and CDR composition biases enrich binder functionality landscapes. J Mol Biol401,84-96.
  5. Higham, EM, Shen, CH, Wittrup, KD, Chen, J (2010). Cutting edge: delay and reversal of T cell tolerance by intratumoral injection of antigen-loaded dendritic cells in an autochthonous tumor model. J Immunol184,5954-8.
  6. Higham, EM, Wittrup, KD, Chen, J (2010). Activation of tolerogenic dendritic cells in the tumor draining lymph nodes by CD8+ T cells engineered to express CD40 ligand. J Immunol184,3394-400.
  7. Hackel, BJ, Wittrup, KD (2010). The full amino acid repertoire is superior to serine/tyrosine for selection of high affinity immunoglobulin G binders from the fibronectin scaffold. Protein Eng Des Sel23,211-9.
  8. Orcutt, KD, Ackerman, ME, Cieslewicz, M, Quiroz, E, Slusarczyk, AL, Frangioni, JV, Wittrup, KD (2010). A modular IgG-scFv bispecific antibody topology. Protein Eng Des Sel23,221-8.

2009

  1. Orcutt, KD, Ackerman, ME, Cieslewicz, M, Quiroz, E, Slusarczyk, AL, Frangioni, JV, Wittrup, KD (2010). A modular IgG-scFv bispecific antibody topology. Protein Eng Des Sel23,221-8.
  2. Schmidt, MM, Wittrup, KD (2009). A modeling analysis of the effects of molecular size and binding affinity on tumor targeting. Mol Cancer Ther8,2861-71.
  3. Liu, DV, Maier, LM, Hafler, DA, Wittrup, KD . Engineered interleukin-2 antagonists for the inhibition of regulatory T cells. J Immunother32,887-94.
  4. Tasumi, S, Velikovsky, CA, Xu, G, Gai, SA, Wittrup, KD, Flajnik, MF, Mariuzza, RA, Pancer, Z (2009). High-affinity lamprey VLRA and VLRB monoclonal antibodies. Proc Natl Acad Sci U S A106,12891-6.
  5. Rakestraw, JA, Sazinsky, SL, Piatesi, A, Antipov, E, Wittrup, KD (2009). Directed evolution of a secretory leader for the improved expression of heterologous proteins and full-length antibodies in Saccharomyces cerevisiae. Biotechnol Bioeng103,1192-201.
  6. Ackerman, M, Levary, D, Tobon, G, Hackel, B, Orcutt, KD, Wittrup, KD . Highly avid magnetic bead capture: an efficient selection method for de novo protein engineering utilizing yeast surface display. Biotechnol Prog25,774-83.
  7. Maier, LM, Anderson, DE, Severson, CA, Baecher-Allan, C, Healy, B, Liu, DV, Wittrup, KD, De Jager, PL, Hafler, DA (2009). Soluble IL-2RA levels in multiple sclerosis subjects and the effect of soluble IL-2RA on immune responses. J Immunol182,1541-7.

2008

  1. Sazinsky, SL, Ott, RG, Silver, NW, Tidor, B, Ravetch, JV, Wittrup, KD (2008). Aglycosylated immunoglobulin G1 variants productively engage activating Fc receptors. Proc Natl Acad Sci U S A105,20167-72.
  2. Antipov, E, Cho, AE, Wittrup, KD, Klibanov, AM (2008). Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method. Proc Natl Acad Sci U S A105,17694-9.
  3. Bai, A, Higham, E, Eisen, HN, Wittrup, KD, Chen, J (2008). Rapid tolerization of virus-activated tumor-specific CD8+ T cells in prostate tumors of TRAMP mice. Proc Natl Acad Sci U S A105,13003-8.
  4. Ackerman, ME, Pawlowski, D, Wittrup, KD (2008). Effect of antigen turnover rate and expression level on antibody penetration into tumor spheroids. Mol Cancer Ther7,2233-40.
  5. Hackel, BJ, Kapila, A, Wittrup, KD (2008). Picomolar affinity fibronectin domains engineered utilizing loop length diversity, recursive mutagenesis, and loop shuffling. J Mol Biol381,1238-52.
  6. Howland, SW, Tsuji, T, Gnjatic, S, Ritter, G, Old, LJ, Wittrup, KD (2008). Inducing efficient cross-priming using antigen-coated yeast particles. J Immunother31,607-19.
  7. Thurber, GM, Schmidt, MM, Wittrup, KD (2008). Antibody tumor penetration: transport opposed by systemic and antigen-mediated clearance. Adv Drug Deliv Rev60,1421-34.
  8. Thurber, GM, Wittrup, KD (2008). Quantitative spatiotemporal analysis of antibody fragment diffusion and endocytic consumption in tumor spheroids. Cancer Res68,3334-41.
  9. Howarth, M, Liu, W, Puthenveetil, S, Zheng, Y, Marshall, LF, Schmidt, MM, Wittrup, KD, Bawendi, MG, Ting, AY (2008). Monovalent, reduced-size quantum dots for imaging receptors on living cells. Nat Methods5,397-9.
  10. Schmidt, MM, Thurber, GM, Wittrup, KD (2008). Kinetics of anti-carcinoembryonic antigen antibody internalization: effects of affinity, bivalency, and stability. Cancer Immunol Immunother57,1879-90.
  11. Ackerman, ME, Chalouni, C, Schmidt, MM, Raman, VV, Ritter, G, Old, LJ, Mellman, I, Wittrup, KD (2008). A33 antigen displays persistent surface expression. Cancer Immunol Immunother57,1017-27.
  12. Howland, SW, Wittrup, KD (2008). Antigen release kinetics in the phagosome are critical to cross-presentation efficiency. J Immunol180,1576-83.
  13. Thurber, GM, Schmidt, MM, Wittrup, KD (2008). Factors determining antibody distribution in tumors. Trends Pharmacol Sci29,57-61.

2007

  1. Yeung, YA, Finney, AH, Koyrakh, IA, Lebowitz, MS, Ghanbari, HA, Wands, JR, Wittrup, KD (2007). Isolation and characterization of human antibodies targeting human aspartyl (asparaginyl) beta-hydroxylase. Hum Antibodies16,163-76.
  2. Lipovsek, D, Antipov, E, Armstrong, KA, Olsen, MJ, Klibanov, AM, Tidor, B, Wittrup, KD (2007). Selection of horseradish peroxidase variants with enhanced enantioselectivity by yeast surface display. Chem Biol14,1176-85.
  3. Lippow, SM, Wittrup, KD, Tidor, B (2007). Computational design of antibody-affinity improvement beyond in vivo maturation. Nat Biotechnol25,1171-6.
  4. Gai, SA, Wittrup, KD (2007). Yeast surface display for protein engineering and characterization. Curr Opin Struct Biol17,467-73.
  5. Campbell, CJ, Wittrup, KD (2007). Protein technologies. Curr Opin Biotechnol18,293-4.
  6. Thurber, GM, Zajic, SC, Wittrup, KD (2007). Theoretic criteria for antibody penetration into solid tumors and micrometastases. J Nucl Med48,995-9.
  7. Lipovsek, D, Lippow, SM, Hackel, BJ, Gregson, MW, Cheng, P, Kapila, A, Wittrup, KD (2007). Evolution of an interloop disulfide bond in high-affinity antibody mimics based on fibronectin type III domain and selected by yeast surface display: molecular convergence with single-domain camelid and shark antibodies. J Mol Biol368,1024-41.
  8. Krauland, EM, Peelle, BR, Wittrup, KD, Belcher, AM (2007). Peptide tags for enhanced cellular and protein adhesion to single-crystalline sapphire. Biotechnol Bioeng97,1009-20.

2006

  1. Chao, G, Lau, WL, Hackel, BJ, Sazinsky, SL, Lippow, SM, Wittrup, KD (2006). Isolating and engineering human antibodies using yeast surface display. Nat Protoc1,755-68.
  2. Swers, JS, Widom, A, Phan, U, Springer, TA, Wittrup, KD (2006). A high affinity human antibody antagonist of P-selectin mediated rolling. Biochem Biophys Res Commun350,508-13.
  3. Rakestraw, JA, Baskaran, AR, Wittrup, KD . A flow cytometric assay for screening improved heterologous protein secretion in yeast. Biotechnol Prog22,1200-8.
  4. Cochran, JR, Kim, YS, Lippow, SM, Rao, B, Wittrup, KD (2006). Improved mutants from directed evolution are biased to orthologous substitutions. Protein Eng Des Sel19,245-53.
  5. Colby, DW, Cassady, JP, Lin, GC, Ingram, VM, Wittrup, KD (2006). Stochastic kinetics of intracellular huntingtin aggregate formation. Nat Chem Biol2,319-23.
  6. Piatesi, A, Howland, SW, Rakestraw, JA, Renner, C, Robson, N, Cebon, J, Maraskovsky, E, Ritter, G, Old, L, Wittrup, KD et al. (2006). Directed evolution for improved secretion of cancer-testis antigen NY-ESO-1 from yeast. Protein Expr Purif48,232-42.
  7. Sivasubramanian, A, Chao, G, Pressler, HM, Wittrup, KD, Gray, JJ (2006). Structural model of the mAb 806-EGFR complex using computational docking followed by computational and experimental mutagenesis. Structure14,401-14.
  8. Midelfort, KS, Wittrup, KD (2006). Context-dependent mutations predominate in an engineered high-affinity single chain antibody fragment. Protein Sci15,324-34.
  9. Kim, YS, Bhandari, R, Cochran, JR, Kuriyan, J, Wittrup, KD (2006). Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast. Proteins62,1026-35.
  10. Rakestraw, A, Wittrup, KD (2006). Contrasting secretory processing of simultaneously expressed heterologous proteins in Saccharomyces cerevisiae. Biotechnol Bioeng93,896-905.

2005

  1. Peelle, BR, Krauland, EM, Wittrup, KD, Belcher, AM (2005). Probing the interface between biomolecules and inorganic materials using yeast surface display and genetic engineering. Acta Biomater1,145-54.
  2. Rao, BM, Driver, I, Lauffenburger, DA, Wittrup, KD (2005). High-affinity CD25-binding IL-2 mutants potently stimulate persistent T cell growth. Biochemistry44,10696-701.
  3. Peelle, BR, Krauland, EM, Wittrup, KD, Belcher, AM (2005). Design criteria for engineering inorganic material-specific peptides. Langmuir21,6929-33.
  4. Rao, BM, Lauffenburger, DA, Wittrup, KD (2005). Integrating cell-level kinetic modeling into the design of engineered protein therapeutics. Nat Biotechnol23,191-4.

2004

  1. Colby, DW, Chu, Y, Cassady, JP, Duennwald, M, Zazulak, H, Webster, JM, Messer, A, Lindquist, S, Ingram, VM, Wittrup, KD et al. (2004). Potent inhibition of huntingtin aggregation and cytotoxicity by a disulfide bond-free single-domain intracellular antibody. Proc Natl Acad Sci U S A101,17616-21.
  2. Midelfort, KS, Hernandez, HH, Lippow, SM, Tidor, B, Drennan, CL, Wittrup, KD (2004). Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody. J Mol Biol343,685-701.
  3. Rao, BM, Driver, I, Lauffenburger, DA, Wittrup, KD (2004). Interleukin 2 (IL-2) variants engineered for increased IL-2 receptor alpha-subunit affinity exhibit increased potency arising from a cell surface ligand reservoir effect. Mol Pharmacol66,864-9.
  4. Colby, DW, Garg, P, Holden, T, Chao, G, Webster, JM, Messer, A, Ingram, VM, Wittrup, KD (2004). Development of a human light chain variable domain (V(L)) intracellular antibody specific for the amino terminus of huntingtin via yeast surface display. J Mol Biol342,901-12.
  5. Chao, G, Cochran, JR, Wittrup, KD (2004). Fine epitope mapping of anti-epidermal growth factor receptor antibodies through random mutagenesis and yeast surface display. J Mol Biol342,539-50.
  6. Colby, DW, Kellogg, BA, Graff, CP, Yeung, YA, Swers, JS, Wittrup, KD (2004). Engineering antibody affinity by yeast surface display. Methods Enzymol388,348-58.
  7. Graff, CP, Chester, K, Begent, R, Wittrup, KD (2004). Directed evolution of an anti-carcinoembryonic antigen scFv with a 4-day monovalent dissociation half-time at 37 degrees C. Protein Eng Des Sel17,293-304.
  8. Cochran, JR, Kim, YS, Olsen, MJ, Bhandari, R, Wittrup, KD (2004). Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments. J Immunol Methods287,147-58.
  9. Johns, TG, Adams, TE, Cochran, JR, Hall, NE, Hoyne, PA, Olsen, MJ, Kim, YS, Rothacker, J, Nice, EC, Walker, F et al. (2004). Identification of the epitope for the epidermal growth factor receptor-specific monoclonal antibody 806 reveals that it preferentially recognizes an untethered form of the receptor. J Biol Chem279,30375-84.
  10. Swers, JS, Kellogg, BA, Wittrup, KD (2004). Shuffled antibody libraries created by in vivo homologous recombination and yeast surface display. Nucleic Acids Res32,e36.
  11. Coughlan, CM, Walker, JL, Cochran, JC, Wittrup, KD, Brodsky, JL (2004). Degradation of mutated bovine pancreatic trypsin inhibitor in the yeast vacuole suggests post-endoplasmic reticulum protein quality control. J Biol Chem279,15289-97.
  12. Grovender, EA, Kellogg, B, Singh, J, Blom, D, Ploegh, H, Wittrup, KD, Langer, RS, Ameer, GA (2004). Single-chain antibody fragment-based adsorbent for the extracorporeal removal of beta2-microglobulin. Kidney Int65,310-22.
  13. Mohr L, Yeung A, Aloman C, Wittrup D, Wands JR. Antibody-directed therapy for human hepatocellular carcinoma. Gastroenterology. 127(5 Suppl 2):S225-31, 2004.

2003

  1. Rao, BM, Girvin, AT, Ciardelli, T, Lauffenburger, DA, Wittrup, KD (2003). Interleukin-2 mutants with enhanced alpha-receptor subunit binding affinity. Protein Eng16,1081-7.
  2. Bhatia, SK, Swers, JS, Camphausen, RT, Wittrup, KD, Hammer, DA . Rolling adhesion kinematics of yeast engineered to express selectins. Biotechnol Prog19,1033-7.
  3. Orr, BA, Carr, LM, Wittrup, KD, Roy, EJ, Kranz, DM . Rapid method for measuring ScFv thermal stability by yeast surface display. Biotechnol Prog19,631-8.
  4. Graff, CP, Wittrup, KD (2003). Theoretical analysis of antibody targeting of tumor spheroids: importance of dosage for penetration, and affinity for retention. Cancer Res63,1288-96.
  5. Feldhaus, MJ, Siegel, RW, Opresko, LK, Coleman, JR, Feldhaus, JM, Yeung, YA, Cochran, JR, Heinzelman, P, Colby, D, Swers, J et al. (2003). Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library. Nat Biotechnol21,163-70.

2002

  1. Reardon, KF, Lee, KH, Wittrup, KD, Hatzimanikatis, V (2002). Jay Bailey as mentor--the students' perspective. Biotechnol Bioeng79,484-9.
  2. Yeung, YA, Wittrup, KD . Quantitative screening of yeast surface-displayed polypeptide libraries by magnetic bead capture. Biotechnol Prog18,212-20.

2001

  1. Wittrup, KD (2001). Protein engineering by cell-surface display. Curr Opin Biotechnol12,395-9.
  2. Kieke, MC, Sundberg, E, Shusta, EV, Mariuzza, RA, Wittrup, KD, Kranz, DM (2001). High affinity T cell receptors from yeast display libraries block T cell activation by superantigens. J Mol Biol307,1305-15.

2000 and earlier

  1. Wittrup, KD (2001). Protein engineering by cell-surface display. Curr Opin Biotechnol12,395-9.
  2. Kieke, MC, Sundberg, E, Shusta, EV, Mariuzza, RA, Wittrup, KD, Kranz, DM (2001). High affinity T cell receptors from yeast display libraries block T cell activation by superantigens. J Mol Biol307,1305-15.
  3. Boder, ET, Wittrup, KD (2000). Yeast surface display for directed evolution of protein expression, affinity, and stability. Methods Enzymol328,430-44.
  4. Wittrup, KD (2000). The single cell as a microplate well. Nat Biotechnol18,1039-40.
  5. Boder, ET, Midelfort, KS, Wittrup, KD (2000). Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity. Proc Natl Acad Sci U S A97,10701-5.
  6. Shusta, EV, Holler, PD, Kieke, MC, Kranz, DM, Wittrup, KD (2000). Directed evolution of a stable scaffold for T-cell receptor engineering. Nat Biotechnol18,754-9.
  7. Boder, ET, Wittrup, KD . Optimal screening of surface-displayed polypeptide libraries. Biotechnol Prog14,55-62.
  8. Holler, PD, Holman, PO, Shusta, EV, O'Herrin, S, Wittrup, KD, Kranz, DM (2000). In vitro evolution of a T cell receptor with high affinity for peptide/MHC. Proc Natl Acad Sci U S A97,5387-92.
  9. Bannister, SJ, Wittrup, KD (2000). Glutathione excretion in response to heterologous protein secretion in Saccharomyces cerevisiae. Biotechnol Bioeng68,389-95.
  10. VanAntwerp, JJ, Wittrup, KD . Fine affinity discrimination by yeast surface display and flow cytometry. Biotechnol Prog16,31-7.
  11. Woycechowsky, KJ, Wittrup, KD, Raines, RT (1999). A small-molecule catalyst of protein folding in vitro and in vivo. Chem Biol6,871-9.
  12. Wittrup, KD (1999). Phage on display. Trends Biotechnol17,423-4.
  13. Shusta, EV, Kieke, MC, Parke, E, Kranz, DM, Wittrup, KD (1999). Yeast polypeptide fusion surface display levels predict thermal stability and soluble secretion efficiency. J Mol Biol292,949-56.
  14. Becker, F, Block-Alper, L, Nakamura, G, Harada, J, Wittrup, KD, Meyer, DI (1999). Expression of the 180-kD ribosome receptor induces membrane proliferation and increased secretory activity in yeast. J Cell Biol146,273-84.
  15. Kieke, MC, Shusta, EV, Boder, ET, Teyton, L, Wittrup, KD, Kranz, DM (1999). Selection of functional T cell receptor mutants from a yeast surface-display library. Proc Natl Acad Sci U S A96,5651-6.
  16. Shusta, EV, VanAntwerp, J, Wittrup, KD (1999). Biosynthetic polypeptide libraries. Curr Opin Biotechnol10,117-22.
  17. Arnold, CE, Parekh, RN, Yang, W, Wittrup, KD (1998). Leader peptide efficiency correlates with signal recognition particle dependence in Saccharomyces cerevisiae. Biotechnol Bioeng59,286-93.
  18. VanAntwerp, JJ, Wittrup, KD (1998). Thermodynamic characterization of affinity maturation: the D1.3 antibody and a higher-affinity mutant. J Mol Recognit11,10-3.
  19. Cho, BK, Kieke, MC, Boder, ET, Wittrup, KD, Kranz, DM (1998). A yeast surface display system for the discovery of ligands that trigger cell activation. J Immunol Methods220,179-88.
  20. Kowalski, JM, Kelly, RM, Konisky, J, Clark, DS, Wittrup, KD (1998). Purification and functional characterization of a chaperone from Methanococcus jannaschii. Syst Appl Microbiol21,173-8.
  21. Shusta, EV, Raines, RT, Plückthun, A, Wittrup, KD (1998). Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments. Nat Biotechnol16,773-7.
  22. Kowalski, JM, Parekh, RN, Mao, J, Wittrup, KD (1998). Protein folding stability can determine the efficiency of escape from endoplasmic reticulum quality control. J Biol Chem273,19453-8.
  23. Kieke, MC, Cho, BK, Boder, ET, Kranz, DM, Wittrup, KD (1997). Isolation of anti-T cell receptor scFv mutants by yeast surface display. Protein Eng10,1303-10.
  24. Kowalski, JM, Parekh, RN, Wittrup, KD (1998). Secretion efficiency in Saccharomyces cerevisiae of bovine pancreatic trypsin inhibitor mutants lacking disulfide bonds is correlated with thermodynamic stability. Biochemistry37,1264-73.
  25. Boder, ET, Wittrup, KD (1997). Yeast surface display for screening combinatorial polypeptide libraries. Nat Biotechnol15,553-7.
  26. Parekh, RN, Wittrup, KD . Expression level tuning for optimal heterologous protein secretion in Saccharomyces cerevisiae. Biotechnol Prog13,117-22.
  27. Zabriskie, DW, Wittrup, KD (1996). Biochemical engineering IX-interdisciplinary foundations for creating new biotechnology: II. Biotechnol Bioeng52,183.
  28. Zabriskie, DW, Wittrup, KD (1996). Biochemical engineering IX-interdisciplinary foundations for creating new biotechnology: I. Biotechnol Bioeng52,1-2.
  29. Robinson, AS, Bockhaus, JA, Voegler, AC, Wittrup, KD (1996). Reduction of BiP levels decreases heterologous protein secretion in Saccharomyces cerevisiae. J Biol Chem271,10017-22.
  30. Parekh, RN, Shaw, MR, Wittrup, KD . An integrating vector for tunable, high copy, stable integration into the dispersed Ty delta sites of Saccharomyces cerevisiae. Biotechnol Prog12,16-21.
  31. Wittrup, KD (1995). Disulfide bond formation and eukaryotic secretory productivity. Curr Opin Biotechnol6,203-8.
  32. Robinson, AS, Wittrup, KD . Constitutive overexpression of secreted heterologous proteins decreases extractable BiP and protein disulfide isomerase levels in Saccharomyces cerevisiae. Biotechnol Prog11,171-7.
  33. Arnold, CE, Wittrup, KD (1994). The stress response to loss of signal recognition particle function in Saccharomyces cerevisiae. J Biol Chem269,30412-8.
  34. Wittrup, KD, Robinson, AS, Parekh, RN, Forrester, KJ (1994). Existence of an optimum expression level for secretion of foreign proteins in yeast. Ann N Y Acad Sci745,321-30.
  35. Wittrup, KD, Westerman, RJ, Desai, R (1994). Fluorescence array detector for large-field quantitative fluorescence cytometry. Cytometry16,206-13.
  36. Robinson, AS, Hines, V, Wittrup, KD (1994). Protein disulfide isomerase overexpression increases secretion of foreign proteins in Saccharomyces cerevisiae. Biotechnology (N Y)12,381-4.
  37. Wittrup, KD, Weber, AL, Tsai, P (1993). Microencapsulation selection for isolation of yeast mutants with increased secretion of Aspergillus awamori glucoamylase. Biotechnol Bioeng42,351-6.
  38. Shaw, MR, Schiek, R, Wittrup, KD . Stochastic stimulation of 2 mu multicopy plasmid amplification in yeast. Biotechnol Prog9,160-5.
  39. Wittrup, KD, Bailey, JE (1991). Comments on a segregated model of recombinant cultures: authors' reply. Biotechnol Bioeng38,1364-5.
  40. Wittrup, KD, Bailey, JE, Ratzkin, B, Patel, A (1990). Propagation of an amplifiable recombinant plasmid in Saccharomyces cerevisiae: flow cytometry studies and segregated modeling. Biotechnol Bioeng35,565-77.
  41. Wittrup, KD, Bailey, JE (1990). Mathematical modeling of a single-cell enzyme assay. Biotechnol Bioeng35,525-32.
  42. Meilhoc, E, Wittrup, KD, Bailey, JE (1989). Application of flow cytometric measurement of surface IgG in kinetic analysis of monoclonal antibody synthesis and secretion by murine hybridoma cells. J Immunol Methods121,167-74.
  43. Budd, ME, Wittrup, KD, Bailey, JE, Campbell, JL (1989). DNA polymerase I is required for premeiotic DNA replication and sporulation but not for X-ray repair in Saccharomyces cerevisiae. Mol Cell Biol9,365-76.
  44. Wittrup, KD, Bailey, JE (1988). A single-cell assay of beta-galactosidase activity in Saccharomyces cerevisiae. Cytometry9,394-404.
  45. Wittrup, KD, Bailey, JE (1988). A segregated model of recombinant multicopy plasmid propagation. Biotechnol Bioeng31,304-10.
  46. Wittrup, KD, Bailey, JE (1988). A mathematical model of recombinational amplification of the 2 mu plasmid in the yeast Saccharomyces cerevisiae. J Theor Biol130,481-92.